pH gradients in immobilized amidases and their influence on rates and yields of beta-lactam hydrolysis.

@article{Spiess1999pHGI,
  title={pH gradients in immobilized amidases and their influence on rates and yields of beta-lactam hydrolysis.},
  author={Antje Christine Spiess and Ralf C. Schlothauer and Joerg Hinrichs and Babbette Scheidat and Volker Kasche},
  journal={Biotechnology and bioengineering},
  year={1999},
  volume={62 3},
  pages={267-77}
}
The pH gradients developing within immobilized biocatalysts during hydrolysis of penicillin G and glutaryl-7-aminocephalosporanic acid have been estimated both theoretically and experimentally. For the latter a fluorimetric method for the direct measurement of the average pH value within the carrier during reaction has been developed using the pH-dependent fluorescence intensity of an enzyme-bound fluorophore determined with a fiber bundle. The theoretical calculations were based on a model for… CONTINUE READING

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