pH gradient elution of human IgG1, IgG2 and IgG4 from protein A-sepharose.

@article{Duhamel1979pHGE,
  title={pH gradient elution of human IgG1, IgG2 and IgG4 from protein A-sepharose.},
  author={R. C. Duhamel and Peter H. Schur and Klaus Brendel and Elias Meezan},
  journal={Journal of immunological methods},
  year={1979},
  volume={31 3-4},
  pages={211-7}
}
Pooled human serum having a normal IgG subclass content was chromatographed on a column of protein A-Sepharose. The immunoglobulins that bound to the column at pH 7.0 were eluted with a pH gradient generated by 3 equal volumes of citrate/phosphate buffer at pH 5.0, 4.5 and 2.2. The elution pattern consisted of two major overlapping peaks centered 0.4 pH units apart in the pH gradient; on average the first peak centered at pH 4.7, and the second centered at pH 4.3. Upon second passage of each… CONTINUE READING