pH effects on the structure of the inner histones.

  title={pH effects on the structure of the inner histones.},
  author={Andrew P. Butler and Donald E Olins},
  journal={Biochimica et biophysica acta},
  volume={698 2},
The structure of the inner histone complex extracted from chicken erythrocyte chromatin with 2 M NaCl has been studied as a function of pH. At pH 6, the complex dissociates to (H3-H4)2 tetramer and H2A.H2B dimer, with little change in alpha-helix content (as monitored by circular dichroism at 222 mm). Although the circular dichroism of tyrosyl side chains is also largely unchanged by the dissociation, measurements of intrinsic fluorescence do suggest a change in the environment of one or more… CONTINUE READING

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