pH-dependent mechanism in the catalysis of prolyl endopeptidase from pig muscle.

@article{Polgar1991pHdependentMI,
  title={pH-dependent mechanism in the catalysis of prolyl endopeptidase from pig muscle.},
  author={László Polgár},
  journal={European journal of biochemistry},
  year={1991},
  volume={197 2},
  pages={
          441-7
        }
}
  • L. Polgár
  • Published 1991
  • Chemistry, Medicine
  • European journal of biochemistry
Prolyl endopeptidase, an enzyme exhibiting high specificity towards the Pro-Xaa bond, is thought to play an important role in the metabolism of biologically active peptides. (a) It has been found that pig muscle is an appropriate source for the preparation of a reasonable quantity of enzyme. Thus, 1 kg muscle yields 1-1.5 mg enzyme, homogeneous by fast protein liquid chromatography. (b) Bulky reagents reacting with cysteine residues inactivate the enzyme almost completely, whereas the small… Expand
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