pH-dependence for binding a single nitrite ion to each type-2 copper centre in the copper-containing nitrite reductase of Alcaligenes xylosoxidans.

@article{Abraham1997pHdependenceFB,
  title={pH-dependence for binding a single nitrite ion to each type-2 copper centre in the copper-containing nitrite reductase of Alcaligenes xylosoxidans.},
  author={Zelda H.L. Abraham and Barry E. Smith and Barry D. Howes and D. Jordan Lowe and Robert R. Eady},
  journal={The Biochemical journal},
  year={1997},
  volume={324 ( Pt 2)},
  pages={511-6}
}
The first quantitative characterization of the interaction of NO2(-) with the Cu-containing dissimilatory nitrite reductase (NiR) of Alcaligenes xylosoxidans using steady-state kinetics, equilibrium gel filtration and EPR spectroscopy is described. Each molecule of this protein consists of three equivalent subunits, each containing a type-1 Cu atom and also a type-2 Cu atom at each subunit interface. Enzyme activity increased in a biphasic manner with decreasing pH, having an optimum at pH 5.2… CONTINUE READING