pH Transitions in ion-exchange systems: role in the development of a cation-exchange process for a recombinant protein.

@article{Ghose2002pHTI,
  title={pH Transitions in ion-exchange systems: role in the development of a cation-exchange process for a recombinant protein.},
  author={Sanchayita Ghose and Thomas M McNerney and Brian C Hubbard},
  journal={Biotechnology progress},
  year={2002},
  volume={18 3},
  pages={530-7}
}
Unexpected transient changes in effluent pH can occur during ion-exchange chromatography. Such changes can occur even if a column that is equilibrated with a buffer receives another solution in the same buffer and of the same pH but of a different salt concentration. An attempt is made to understand the basis for this phenomenon and apply it to the process purification of a recombinant protein on a strong cation-exchange resin. Incomplete column equilibration was eliminated as a possible cause… CONTINUE READING