pH-Responsive quencher-free molecular beacon systems containing 2'-deoxyuridine units labeled with fluorene derivatives.

@article{Lee2017pHResponsiveQM,
  title={pH-Responsive quencher-free molecular beacon systems containing 2'-deoxyuridine units labeled with fluorene derivatives.},
  author={Ji Won Lee and Ye-Seul Son and Jun Yeon Hwang and YooJin Park and Gil Tae Hwang},
  journal={Organic & biomolecular chemistry},
  year={2017},
  volume={15 34},
  pages={
          7165-7172
        }
}
Three fluorescent nucleosides-UFL, UAF, and UDAF, containing fluorene, 2-aminofluorene, and 2-dimethylaminofluorene units, respectively, covalently attached to 2'-deoxyuridine-have been incorporated into the central positions of oligodeoxynucleotides (ODNs) to examine the effects of their flanking bases (FBs) and pH on the emission properties upon hybridization with fully matched and single-base-mismatched targets. The ODN containing UFL and cytosine-FBs in the pH range from 5.5 to 8.0 and the… CONTINUE READING
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