pH-Dependent channel activity of heterologously-expressed main intrinsic protein (MIP) from rat lens.

@article{Drake2002pHDependentCA,
  title={pH-Dependent channel activity of heterologously-expressed main intrinsic protein (MIP) from rat lens.},
  author={K Dawn Drake and Diana Schuette and Ana B. Chepelinsky and Tim J. C. Jacob and M. James C. Crabbe},
  journal={FEBS letters},
  year={2002},
  volume={512 1-3},
  pages={199-204}
}
Wild-type rat lens main intrinsic protein (MIP) was heterologously expressed in the membrane of Spodoptera frugiperda (Sf21) cells using the baculovirus expression system and in mouse erythroid leukaemia cells (MEL C88). Both MEL and Sf21 cell lines expressing wild-type MIP were investigated for the conductance of ions using a whole cell patch clamp technique. An increase in conductance was seen in both expression systems, particularly on lowering the pH to 6.3. In Sf21 cells, addition of… CONTINUE READING