pDUAL: a transposon-based cosmid cloning vector for generating nested deletions and DNA sequencing templates in vivo.

@article{Wang1993pDUALAT,
  title={pDUAL: a transposon-based cosmid cloning vector for generating nested deletions and DNA sequencing templates in vivo.},
  author={Guolun Wang and R. W. Blakesley and Douglas E. Berg and Claire M. Berg},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1993},
  volume={90 16},
  pages={7874-8}
}
We describe a transposon gamma delta-containing cosmid cloning vector, pDUAL (previously called pJANUS), and demonstrate an efficient strategy for isolating nested deletions in both large-scale and small-scale DNA sequencing efforts. This "deletion factory" strategy takes advantage of the ability of gamma delta (Tn1000) to generate deletions that extend from an end of the transposon into adjacent DNA when gamma delta transposes to new sites in the same DNA molecule. pDUAL contains the… CONTINUE READING