pBECKS2000: a novel plasmid series for the facile creation of complex binary vectors, which incorporates “clean-gene” facilities

@article{McCormac1999pBECKS2000AN,
  title={pBECKS2000: a novel plasmid series for the facile creation of complex binary vectors, which incorporates “clean-gene” facilities},
  author={A. C. McCormac and M. C. Elliott and D.-F. Chen},
  journal={Molecular and General Genetics MGG},
  year={1999},
  volume={261},
  pages={226-235}
}
  • A. C. McCormac, M. C. Elliott, D.-F. Chen
Abstract A new plasmid series has been created for Agrobacterium-mediated plant transformation. The pBECKS2000 series of binary vectors exploits the Cre/loxP site-specific recombinase system to facilitate the construction of complex T-DNA vectors. The new plasmids enable the rapid generation of T-DNA vectors in which multiple genes are linked, without relying on the availability of purpose-built cassette systems or demanding complex, and therefore inefficient, ligation reactions. The vectors… CONTINUE READING

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