p53 mutations in human bladder cancer.

  title={p53 mutations in human bladder cancer.},
  author={W C Kusser and Xiong-ying Miao and Barry W. Glickman and Joan Friedland and Nathaniel Rothman and George P. Hemstreet and J Mellot and Daniel C. Swan and Paul A Schulte and Richard B Hayes},
  journal={Environmental and molecular mutagenesis},
  volume={24 3},
Mutations in the tumor suppressor gene p53 play an important role in carcinogenesis and tumor progression. To assess the status of p53 from genomic DNA from bladder cancer samples a two stage polymerase chain reaction was employed. The technique provided material for subsequent detection of mutations by Single Strand Conformation Polymorphism (SSCP) analysis followed by DNA sequence analysis. SSCP analysis of exons 5 to 9 of p53 was performed using fragments from PCR end-labeled with 32P… CONTINUE READING