p53-induced RING-H2 protein, a novel marker for poor survival in hepatocellular carcinoma after hepatic resection.

@article{Wang2009p53inducedRP,
  title={p53-induced RING-H2 protein, a novel marker for poor survival in hepatocellular carcinoma after hepatic resection.},
  author={Xiao-Min Wang and Lian-yue Yang and Lei Guo and Chun Chieh Fan and Fan Wu},
  journal={Cancer},
  year={2009},
  volume={115 19},
  pages={4554-63}
}
BACKGROUND Currently, the role of p53-induced RING-H2 protein (PIRH2) in the development of hepatocellular carcinoma (HCC) remains unknown. The objective of this retrospective study was to investigate the expression of PIRH2 and its relation to prognosis in patients with HCC after hepatic resection. METHODS Reverse transcriptase-polymerase chain reaction (RT-PCR), quantitative real-time RT-PCR, and Western blot analyses were used to detect expression levels of PIRH2 in 30 samples of HCC… CONTINUE READING

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Reverse transcriptase - polymerase chain reaction ( RT - PCR ) , quantitative real - time RT - PCR , and Western blot analyses were used to detect expression levels of PIRH2 in 30 samples of HCC tissue and paracarcinomatous liver tissue ( PCLT ) and in 5 samples of normal liver tissue ( NL ) .
Reverse transcriptase - polymerase chain reaction ( RT - PCR ) , quantitative real - time RT - PCR , and Western blot analyses were used to detect expression levels of PIRH2 in 30 samples of HCC tissue and paracarcinomatous liver tissue ( PCLT ) and in 5 samples of normal liver tissue ( NL ) .
Reverse transcriptase - polymerase chain reaction ( RT - PCR ) , quantitative real - time RT - PCR , and Western blot analyses were used to detect expression levels of PIRH2 in 30 samples of HCC tissue and paracarcinomatous liver tissue ( PCLT ) and in 5 samples of normal liver tissue ( NL ) .
Reverse transcriptase - polymerase chain reaction ( RT - PCR ) , quantitative real - time RT - PCR , and Western blot analyses were used to detect expression levels of PIRH2 in 30 samples of HCC tissue and paracarcinomatous liver tissue ( PCLT ) and in 5 samples of normal liver tissue ( NL ) .
Reverse transcriptase - polymerase chain reaction ( RT - PCR ) , quantitative real - time RT - PCR , and Western blot analyses were used to detect expression levels of PIRH2 in 30 samples of HCC tissue and paracarcinomatous liver tissue ( PCLT ) and in 5 samples of normal liver tissue ( NL ) .
Reverse transcriptase - polymerase chain reaction ( RT - PCR ) , quantitative real - time RT - PCR , and Western blot analyses were used to detect expression levels of PIRH2 in 30 samples of HCC tissue and paracarcinomatous liver tissue ( PCLT ) and in 5 samples of normal liver tissue ( NL ) .
Reverse transcriptase - polymerase chain reaction ( RT - PCR ) , quantitative real - time RT - PCR , and Western blot analyses were used to detect expression levels of PIRH2 in 30 samples of HCC tissue and paracarcinomatous liver tissue ( PCLT ) and in 5 samples of normal liver tissue ( NL ) .
Reverse transcriptase - polymerase chain reaction ( RT - PCR ) , quantitative real - time RT - PCR , and Western blot analyses were used to detect expression levels of PIRH2 in 30 samples of HCC tissue and paracarcinomatous liver tissue ( PCLT ) and in 5 samples of normal liver tissue ( NL ) .
Reverse transcriptase - polymerase chain reaction ( RT - PCR ) , quantitative real - time RT - PCR , and Western blot analyses were used to detect expression levels of PIRH2 in 30 samples of HCC tissue and paracarcinomatous liver tissue ( PCLT ) and in 5 samples of normal liver tissue ( NL ) .
Reverse transcriptase - polymerase chain reaction ( RT - PCR ) , quantitative real - time RT - PCR , and Western blot analyses were used to detect expression levels of PIRH2 in 30 samples of HCC tissue and paracarcinomatous liver tissue ( PCLT ) and in 5 samples of normal liver tissue ( NL ) .
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