oriT-processing and regulatory roles of TrwA protein in plasmid R388 conjugation.

  title={oriT-processing and regulatory roles of TrwA protein in plasmid R388 conjugation.},
  author={Gabriel Moncali{\'a}n and G Grandoso and Matxalen Llosa and F Sebasti{\'a}n de la Cruz},
  journal={Journal of molecular biology},
  volume={270 2},
TrwA protein was purified from an overproducing Escherichia coli strain and characterized as a 53 kDa tetrameric DNA-binding protein. Gel shift assays showed that TrwA bound specifically to the oriT sequence of plasmid R388. DNAse I footprinting analysis defined two DNA regions within oriT (sites A and B) that were protected by TrwA. At low TrwA concentrations only region A was protected (K(D) = 4 x 10(-8) M) while region B required higher TrwA concentrations (K(D) = 4 x 10(-7) M). As a result… CONTINUE READING


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