ompA gene in the detection of Escherichia coli and other Enterobacteriaceae by nucleic acid sandwich hybridization.

@article{Palva1983ompAGI,
  title={ompA gene in the detection of Escherichia coli and other Enterobacteriaceae by nucleic acid sandwich hybridization.},
  author={Airi M Palva},
  journal={Journal of clinical microbiology},
  year={1983},
  volume={18 1},
  pages={92-100}
}
  • Airi M Palva
  • Published 1983 in Journal of clinical microbiology
The applicability of the DNA sandwich hybridization method to detection of bacterial DNA from crude samples is demonstrated using Escherichia coli as a model. In sandwich hybridization the sample DNA mediates the binding of a labeled probe DNA fragment to a second DNA bound on filter. For this study the DNA reagents were prepared by subclonings from a recombinant plasmid containing the E. coli K-12 ompA gene and an adjacent fragment of E. coli DNA. The 5' half of the ompA gene (738 base pairs… CONTINUE READING

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