n-Alkane oxidation by aPseudomonas

  title={n-Alkane oxidation by aPseudomonas},
  author={G. Thijsse and A. Linden},
  journal={Antonie van Leeuwenhoek},
Summary1.APseudomonas oxidizingn-alkanes adaptively was used in experiments on hexane and heptane degradation.2.The R.Q. of heptane respiration by resting heptane-adapted cells was found to be 0.62. No appreciable accumulation of products except CO2 and H2O seems to occur.3.Alkane oxidation proceeds by oxidation of one terminal methyl group, leading to the corresponding alcohols, aldehydes and fatty acids as indicated by multiple adaption studies.4.Oxidation of intermediate fatty acids by a… Expand
29 Citations

Figures and Tables from this paper

n-alkane oxidation by apseudomonas formation and β-oxidation of intermediate fatty acids
SummaryFrom a culture broth ofPseudomonas aeruginosa (KSLA strain 473) grown on heptane as the sole source of carbon, fatty acids could be isolated after a period of decreased oxygen supply. TheExpand
Pathways of hydrocarbon dissimilation by aPseudomonas as revealed by chloramphenicol
Attack on the saturated end of the molecule seems well in line with the assumption that alkane oxidation by these bacteria is effected by oxygen transferring enzymes operating on a methyl group, as opposed to the action of a dehydrogenase and formation of an α-olefin as the intermediate. Expand
The oxidation of α-olefins by aPseudomonas reactions involving the double bond
The preponderant pathway of octene-1 degradation by octane- and octene-1-grownPseudomonas aeruginosa cells (strain 473) starts with oxidation of the methyl group.In addition, with both types of cellsExpand
Iso-alkane oxidation by aPseudomonas Part I.—Metabolism of 2-methylhexane
SummaryPseudomonas cells adapted to 2-methylhexane oxidize 5-methylhexanoic andiso-valeric acids rapidly. The oxidation rates of 2-methylhexanoic and propionic acids are appreciably lower. It can beExpand
Induction of alkane-oxidizing andα-olefin-epoxidizing enzymes by a non-hydrocarbon in aPseudomonas
A strain ofPseudomonas aeruginosa could be induced to oxidizen-paraffins and to epoxidizeα-olefins by treating peptone-grown cells with 1,6-hexanediol or by growing them on this substrate. Of someExpand
Genetics of alkane oxidation byPseudomonas oleovorans
The genetics and enzymology of alkane metabolism have been investigated in depth for Pseudomonas oleovorans, which is able to oxidize C5-C12 n-alkanes by virtue of two gene regions, localized on the OCT-plasmid. Expand
Degradation ofn-alkane-1-sulfonates byPseudomonas
We have isolated two strains ofPseudomonas, AJ 1 and AJ 2, growing on the C4–C7 and the C8–C12n-alkane-1-sulfonates, respectively, as the only source of their carbon and energy.The alkane sulfonatesExpand
Non-stereospecific oxidation of DL-3-methylheptane by aPseudomonas
The conclusion that 3- methylheptane oxidation is non-stereospecific applies also to 3-methylhexane, but cannot safely be generalized to include other alkanes (and micro-organisms) as well. Expand
Dissimilation of 1,6-hexanediol and 1,8-octanediol by a hydrocarbon-oxidizingPseudomonas
  • A. Linden
  • Chemistry
  • Antonie van Leeuwenhoek
  • 2005
Abstract1,6-Hexanediol is dissimilated by restingPseudomonas aeruginosa cells to give 6-hydroxyhexanoate, which is subject to β- as well as to ω-oxidation.β-Oxidation leads to 4-hydroxybutyrate,Expand
Occurrence of inducible and NAD(P)-independent primary alcohol dehydrogenases in an alkane-oxidizingPseudomonas
Pseudomonas aeruginosa constitutively contains a soluble NADP-linked dehydrogenase active towards primary alcohols and at least two NAD(P)-independent primary alcohol dehydrogenases can be induced by growing this strain onPrimary alcohols,α,ω-diols orn-alkanes. Expand


Evidence for β-Oxidation in the Metabolism of Saturated Aliphatic Hydrocarbons by Soil Species of Nocardia
MEMBERS of the genus Nocardia can use certain long-chain saturated aliphatic hydrocarbons (C10–C18) as sole carbon and energy source1, but very little information is available about the mechanism byExpand
Respiration studies of a Micrococcus capable of oxidizing hydrocarbons.
  • J. O. Harris
  • Chemistry, Medicine
  • Archives of biochemistry and biophysics
  • 1957
The highly active enzyme system in a Micrococcus species capable of oxidizing a number of hydrocarbons was shown to be independent of Hydrocarbons in the growth medium, which may explain why decane is less readily oxidized than octane and dodecane. Expand
This communication includes results which suggest that two pathways operate in N. corallina for the oxidation of propionic acid. Expand
The Oxidation of Hydrocarbons by Soil Bacteria I. Morphological and Biochemical Properties of a Soil Diphtheroid Utilizing Hydrocarbons
(1) A. description is given of the morphology and hiochomical propertios of a soil hacterium, (JorynPi)(lctcl'iwn sp., ahle to oxidize aliphatic hydl'ocm·bolls.
Metabolism of propionic acid in animal tissues. I. Enzymatic conversion of propionate to succinate.
Problems of bacterial oxidative metabolism.
  • R. Stanier
  • Biology, Medicine
  • Bacteriological reviews
  • 1950