mproved poliovirus d-antigen yields by application of different Vero ell cultivation methods

  • E. Thomassena, Olaf Rubingha, René H. Wijffelsb, Leo A. van der Pola, A. M. Bakkera
  • Published 2014

Abstract

Vero cells were grown adherent to microcarriers (Cytodex 1; 3 g L−1) using animal component free media in stirred-tank type bioreactors. Different strategies for media refreshment, daily media replacement (semi-batch), continuous media replacement (perfusion) and recirculation of media, were compared with batch cultivation. Cell densities increased using a… (More)

1 Figure or Table

Topics

  • Presentations referencing similar topics