migS, a cis-acting site that affects bipolar positioning of oriC on the Escherichia coli chromosome.

@article{Yamaichi2004migSAC,
  title={migS, a cis-acting site that affects bipolar positioning of oriC on the Escherichia coli chromosome.},
  author={Yoshiharu Yamaichi and Hironori Niki},
  journal={The EMBO journal},
  year={2004},
  volume={23 1},
  pages={221-33}
}
During replication of the Escherichia coli chromosome, the replicated Ori domains migrate towards opposite cell poles, suggesting that a cis-acting site for bipolar migration is located in this region. To identify this cis-acting site, a series of mutants was constructed by splitting subchromosomes from the original chromosome. One mutant, containing a 720 kb subchromosome, was found to be defective in the bipolar positioning of oriC. The creation of deletion mutants allowed the identification… CONTINUE READING