miRNA-125b regulates TNF-α production in CD14+ neonatal monocytes via post-transcriptional regulation.

@article{Huang2012miRNA125bRT,
  title={miRNA-125b regulates TNF-α production in CD14+ neonatal monocytes via post-transcriptional regulation.},
  author={Hsin-Chun Huang and Hong-Ren Yu and Li-Tung Huang and Hui-Chen Huang and Ron-Fu Chen and I-Chun Lin and Chia-Yo Ou and T -Y Hsu and Kuender D. Yang},
  journal={Journal of leukocyte biology},
  year={2012},
  volume={92 1},
  pages={171-82}
}
Neonates, although deficient in cell immunity, frequently reveal sepsis with augmented proinflammatory reactions. Here, we found that neonatal monocytes produced significantly higher TNF-α mRNA and protein than adult monocytes. Assessment of the transcriptional factor found no significant difference of NF-κB p65 level between neonatal and adult monocytes. Addition of Act D to access the half-life of TNF-α mRNA revealed no significant difference of the LPS-induced TNF-α mRNA half-life between… CONTINUE READING