miR-191 promotes tumorigenesis of human colorectal cancer through targeting C/EBPβ

Abstract

MicroRNA-191 (miR-191), a small non-coding RNA, is involved in disease development and cancer diagnosis and prognosis. However, how miR-191 functions in colorectal cancer remains largely unclear. In this study, we show that miR-191 is highly expressed in colon tumor tissues, and that inhibition of miR-191 leads to decreased cell growth, proliferation and tumorigenicity in a xenograft model. Overexpression of miR-191 in colorectal cancer cell lines alters cell cycle progression and cell resistance to 5-Fu induced cell apoptosis. Mechanistic studies demonstrated that miR-191 directly binds to the 3'UTR of the C/EBPβ mRNA and mediates a decrease in the mRNA and protein expression of C/EBPβ. We further showed that C/EBPβ induces growth arrest in a colorectal cancer cell line and that its expression is negatively correlated with the miR-191 level in patient samples. Our findings suggest that miR-191 may be a potential gene therapy target for the treatment of colorectal cancer.

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@inproceedings{Zhang2015miR191PT, title={miR-191 promotes tumorigenesis of human colorectal cancer through targeting C/EBPβ}, author={Xiao-Fei Zhang and Ke-ke Li and Lu Gao and Shang-ze Li and Ke Chen and Jun-Bin Zhang and Di Wang and Rong-Fu Tu and Jin-xiang Zhang and Kaixiong Tao and Guobin Wang and Xiaodong Zhang}, booktitle={Oncotarget}, year={2015} }