mRNA transfection of cervical carcinoma and mesenchymal stem cells mediated by cationic carriers.

@article{Rejman2010mRNATO,
  title={mRNA transfection of cervical carcinoma and mesenchymal stem cells mediated by cationic carriers.},
  author={Joanna Rejman and Geertrui Tavernier and Neda Bavarsad and Joseph Demeester and Stefaan C. De Smedt},
  journal={Journal of controlled release : official journal of the Controlled Release Society},
  year={2010},
  volume={147 3},
  pages={385-91}
}
Messenger RNA encoding luciferase (mLUC) was complexed to the cationic lipids Lipofectamine or DOTAP/DOPE, and to the cationic polymer linear poly(ethyleneimine) (linPEI). The complexes were incubated with HeLa cells and luciferase expression was assessed. The type of non-viral carrier used determined the extent and duration of protein expression. Maximal duration of mRNA expression was about 9 days for Lipofectamine complexes, i.e. not very much shorter than with pDNA polyplexes. Interestingly… CONTINUE READING