mRNA-sequencing whole transcriptome analysis of a single cell on the SOLiD system.

  title={mRNA-sequencing whole transcriptome analysis of a single cell on the SOLiD system.},
  author={Kai Qin Lao and Fuchou Tang and Catalin Barbacioru and Yangzhou Wang and Ellen Nordman and Clarence Lee and N Xu and Xiaohui Wang and Brain Tuch and John P. Bodeau and Asim Siddiqui and M. Azim Surani},
  journal={Journal of biomolecular techniques : JBT},
  volume={20 5},
We have developed a sequencing-based gene expression profiling assay at single-cell resolution by combining a modified single-cell whole transcriptome amplification method with the next generation sequencing technique, the SOLiD system. Using this assay, we have shown that blastomeres in a four-cell stage embryo have similar gene expression, which is compatible with the fact that they have similar developmental potential. We proved that compared with cDNA microarray technique, our single-cell… CONTINUE READING
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