mRNA localization signals can enhance the intracellular effectiveness of hammerhead ribozymes.

@article{Lee1999mRNALS,
  title={mRNA localization signals can enhance the intracellular effectiveness of hammerhead ribozymes.},
  author={Nan Sook Lee and Edouard Bertrand and John J. Rossi},
  journal={RNA},
  year={1999},
  volume={5 9},
  pages={1200-9}
}
Subcellular localization signals for several mRNAs are positioned in their 3' untranslated regions (UTR). We have utilized the human alpha- and beta-actin 3' UTRs as signals for colocalizing hammerhead ribozymes with a lacZtarget mRNA. Ribozyme and target genes containing matched or unmatched 3' UTRs were cotransfected into 12-day-old chicken embryonic myoblast and fibroblast (CEMF) cultures and assayed by in situ hybridization (ISH) using a dual label, antibody sandwich procedure, and dual… CONTINUE READING

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