mRNA distribution and membrane localization of the OAT-K1 organic anion transporter in rat renal tubules.

@article{Masuda1997mRNADA,
  title={mRNA distribution and membrane localization of the OAT-K1 organic anion transporter in rat renal tubules.},
  author={Satohiro Masuda and Hideyuki Saito and Hiroshi Nonoguchi and Kyoji Tomita and Ken-ichi Inui},
  journal={FEBS letters},
  year={1997},
  volume={407 2},
  pages={127-31}
}
OAT-K1, a renal organic anion transporter, which mediates methotrexate uptake, was analyzed for mRNA distribution along microdissected nephron segments and the immunolocalization in isolated plasma membranes from rat kidney. By using a reverse transcription-coupled PCR, OAT-K1 mRNA was detected predominantly in the superficial and juxtamedullary proximal straight tubules. Western blotting with antiserum for OAT-K1 revealed that the transporter protein with the apparent molecular mass of 40 kDa… CONTINUE READING
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