mRNA display design of fibronectin-based intrabodies that detect and inhibit severe acute respiratory syndrome coronavirus nucleocapsid protein.

@article{Liao2009mRNADD,
  title={mRNA display design of fibronectin-based intrabodies that detect and inhibit severe acute respiratory syndrome coronavirus nucleocapsid protein.},
  author={Hsien-hao Sebastian Liao and Connor A. Olson and Seungmin Hwang and Hongyu Deng and Elaine Y. Wong and Ralph S. Baric and Richard W Roberts and Ren Sun},
  journal={The Journal of biological chemistry},
  year={2009},
  volume={284 26},
  pages={
          17512-20
        }
}
The nucleocapsid (N) protein of severe acute respiratory syndrome (SARS) coronavirus plays important roles in both viral replication and modulation of host cell processes. New ligands that target the N protein may thus provide tools to track the protein inside cells, detect interaction hot spots on the protein surface, and discover sites that could be used to develop new anti-SARS therapies. Using mRNA display selection and directed evolution, we designed novel antibody-like protein affinity… CONTINUE READING

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