mRNA and microRNA expression profiles in circulating tumor cells and primary tumors of metastatic breast cancer patients.

@article{Sieuwerts2011mRNAAM,
  title={mRNA and microRNA expression profiles in circulating tumor cells and primary tumors of metastatic breast cancer patients.},
  author={Anieta M. Sieuwerts and Bianca Mostert and Joan B. Bolt-de Vries and Dieter Peeters and Felix E. de Jongh and Jacqueline M L Stouthard and Luc Y Dirix and Peter A van Dam and Anne van Galen and Vanja de Weerd and J. K. Kraan and Petra van der Spoel and Raquel Ram{\'i}rez-Moreno and Carolien H. M. van Deurzen and Marcel Smid and Jack X Yu and John Z Jiang and Yixin Wang and Jan Willem C. Gratama and Stefan Sleijfer and John A. Foekens and John W. M. Martens},
  journal={Clinical cancer research : an official journal of the American Association for Cancer Research},
  year={2011},
  volume={17 11},
  pages={3600-18}
}
PURPOSE Molecular characterization of circulating tumor cells (CTC) holds great promise. Unfortunately, routinely isolated CTC fractions currently still contain contaminating leukocytes, which makes CTC-specific molecular characterization extremely challenging. In this study, we determined mRNA and microRNA (miRNA) expression of potentially CTC-specific genes that are considered to be clinically relevant in breast cancer. EXPERIMENTAL DESIGN CTCs were isolated with the epithelial cell… CONTINUE READING