mRNA-Seq whole-transcriptome analysis of a single cell

@article{Tang2009mRNASeqWA,
  title={mRNA-Seq whole-transcriptome analysis of a single cell},
  author={F. Tang and C{\'a}t{\'a}lin B{\'a}rb{\'a}cioru and Yangzhou Wang and E. Nordman and C. Lee and N. Xu and Xiaohui Wang and J. Bodeau and B. B. Tuch and A. Siddiqui and K. Lao and M. Surani},
  journal={Nature Methods},
  year={2009},
  volume={6},
  pages={377-382}
}
  • F. Tang, Cátálin Bárbácioru, +9 authors M. Surani
  • Published 2009
  • Biology, Medicine
  • Nature Methods
  • Next-generation sequencing technology is a powerful tool for transcriptome analysis. However, under certain conditions, only a small amount of material is available, which requires more sensitive techniques that can preferably be used at the single-cell level. Here we describe a single-cell digital gene expression profiling assay. Using our mRNA-Seq assay with only a single mouse blastomere, we detected the expression of 75% (5,270) more genes than microarray techniques and identified 1,753… CONTINUE READING
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