mDia mediates Rho-regulated formation and orientation of stable microtubules

  title={mDia mediates Rho-regulated formation and orientation of stable microtubules},
  author={Alexander F. Palazzo and Tiffani A. Cook and Arthur S. Alberts and Gregg G. Gundersen},
  journal={Nature Cell Biology},
Rho-GTPase stabilizes microtubules that are oriented towards the leading edge in serum-starved 3T3 fibroblasts through an unknown mechanism. We used a Rho-effector domain screen to identify mDia as a downstream Rho effector involved in microtubule stabilization. Constitutively active mDia or activation of endogenous mDia with the mDia-autoinhibitory domain stimulated the formation of stable microtubules that were capped and oriented towards the wound edge. mDia co-localized with stable… 

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