m6A RNA methylation regulates the fate of endogenous retroviruses

  title={m6A RNA methylation regulates the fate of endogenous retroviruses},
  author={Tomasz Chelmicki and Emeline Roger and Aurelie Teissandier and Sofia Rucli and François Dossin and M. Dura and Camille Fouassier and Sonia Lameiras and D. Bourc’his},
Endogenous retroviruses (ERVs) are abundant and heterogenous groups of integrated retroviral sequences that impact genome regulation and cell physiology throughout their RNA-centered life cycle1. Failure to repress ERVs is associated with cancer, infertility, senescence and neurodegenerative diseases2–4. Here, using an unbiased genome-scale CRISPR knockout screen in mouse embryonic stem cells, we identify m6A RNA methylation as a novel means of ERV restriction. Methylation of ERV mRNAs is… Expand
m6A RNA methylation of major satellite repeat transcripts facilitates chromatin association and RNA:DNA hybrid formation in mouse heterochromatin
It is proposed that m6A modification ofMSR RNA will enhance the functions of MSR repeat transcripts to stabilize mouse heterochromatin and form RNA:DNA hybrids. Expand
Human Endogenous Retrovirus as Therapeutic Targets in Neurologic Disease
This review summarizes the recent advances on the epigenetic mechanisms controlling HERV expression and the pathogenic effects triggered by HERV de-repression and describes new, promising therapies, targeting HERV elements, one of which, temelimab, has completed phase II trials with encouraging results in treating MS. Expand
New insights into the functional role of retrotransposon dynamics in mammalian somatic cells
Recent findings unveiling the regulatory potential of retrotransposons are reviewed, including their role in noncoding RNA transcription, as modulators of mammalian transcriptional and epigenome landscapes, highlighting an unforeseen central role of this neglected portion of the genome both in early development and in adult life. Expand
Endogenous retroviruses in the origins and treatment of cancer
Although ERV expression is associated with cancer, and provisionally with autoimmune and neurodegenerative diseases, ERV-mediated inflammation is being explored as a way to sensitize tumors to immunotherapy and the wider biomedical potential of therapies directed at ERVs is reviewed. Expand
RNA demethylation increases the yield and biomass of rice and potato plants in field trials.
  • Qiong Yu, Shun Liu, +23 authors G. Jia
  • Medicine
  • Nature biotechnology
  • 2021
Modulation of plant RNA m6A methylation is a promising strategy to dramatically improve plant growth and crop yield and stimulates root meristem cell proliferation and tiller bud formation and promotes photosynthetic efficiency and drought tolerance. Expand
Regulatory effect of m6A modification on different viruses
  • Pei-Lun Yu, San-Jie Cao, Rui Wu, Qin Zhao, Qi-Gui Yan
  • Medicine
  • Journal of medical virology
  • 2021
This paper reviews the regulatory effects of m6 A modification on different viruses, and provides a reference for studying the Regulatory effects of RNA epitranscriptomic modification. Expand
Potential role of m6A RNA methylation regulators in osteosarcoma and its clinical prognostic value
  • Hua Liu, Guangzhen Qin, +5 authors Z. Cai
  • Medicine
  • Journal of Orthopaedic Surgery and Research
  • 2021
Background Osteosarcoma is a disease with high mortality in children and adolescents, and metastasis is one of the important clinical features of osteosarcoma. N 6-Methyladenosine (m6A) is the mostExpand
Tumor immunity and immunotherapy: opportunities and challenges
  • 2021
[基金项目] 国家科技重大专项课题资助项目(No.2017ZX10203206-002);国家自然科学基金资助项目(No.81972683);上海市青年科技启明星 计划资助项目(No. 19QA1411300)。 Project supported by the National Science and Technology Major Project (No.Expand
m6A modifications regulate intestinal immunity and rotavirus infection
  • Anmin Wang, Wanyin Tao, +14 authors Shu Zhu
  • Biology
  • 2021
N6-methyladenosine (m6A) is an abundant mRNA modification and affects many biological processes. However, how m6A levels are regulated during physiological or pathological processes such as virusExpand


KAP1 controls endogenous retroviruses in embryonic stem cells
It is shown that KAP1 deletion leads to a marked upregulation of a range of ERVs, in particular IAP elements, in mouse embryonic stem (ES) cells and in early embryos, and that it is enriched at the 5′ untranslated region (5′UTR) of IAP genomes. Expand
Zc3h13 Regulates Nuclear RNA m6A Methylation and Mouse Embryonic Stem Cell Self-Renewal.
It is demonstrated that Zc3h13 plays a critical role in anchoring WTAP, Virilizer, and Hakai in the nucleus to facilitate m6A methylation and to regulate mESC self-renewal. Expand
N6-methyladenosine of chromosome-associated regulatory RNA regulates chromatin state and transcription
  • Jun Liu, Xiaoyang Dou, +8 authors Chuan He
  • Medicine, Chemistry
  • Science
  • 2020
Results reveal that m6A on carRNAs can globally tune chromatin state and transcription and that knockout of the m 6A writer Mettl3 or the nuclear reader Ythdc1 in mouse embryonic stem cells increases chromatin accessibility and activates transcription in anm6A-dependent manner. Expand
m6A-dependent regulation of messenger RNA stability
It is shown that m6A is selectively recognized by the human YTH domain family 2 (YTHDF2) ‘reader’ protein to regulate mRNA degradation and established the role of YTH DF2 in RNA metabolism, showing that binding of Y THDF2 results in the localization of bound mRNA from the translatable pool to mRNA decay sites, such as processing bodies. Expand
A CRISPR knockout screen identifies SETDB1-target retroelement silencing factors in embryonic stem cells.
A genome-wide CRISPR screen cataloged genes which function at different levels in silencing of SETDB1-target retroelements and provides a useful resource for further molecular studies. Expand
m(6)A RNA modification controls cell fate transition in mammalian embryonic stem cells.
Mapping the m( 6)A methylome in mouse and human embryonic stem cells reveals the evolutionary conservation and function of m(6)A, a mark of transcriptome flexibility required for stem cells to differentiate to specific lineages. Expand
N6-methyladenosine regulates the stability of RNA:DNA hybrids in human cells
It is shown that N6-methyladenosine (m6A) modification, contributing to different aspects of messenger RNA metabolism5,6, is detectable on the majority of RNA:DNA hybrids in human pluripotent stem cells, and that m6A regulates accumulation of R-loops, implying a role for this modification in safeguarding genomic stability. Expand
N 6-methyladenosine-dependent regulation of messenger RNA stability
N-methyladenosine (mA) is the most prevalent internal (non-cap) modification present in the messenger RNA of all higher eukaryotes. Although essential to cell viability and development, the exactExpand
m6A mRNA methylation facilitates resolution of naïve pluripotency toward differentiation
It is shown that N6-methyladenosine (m6A), a messenger RNA (mRNA) modification present on transcripts of pluripotency factors, drives this transition from the pluripotent to the differentiated state. Expand
A genome-wide RNAi screen identifies a new transcriptional module required for self-renewal.
A genome-wide siRNA screen in mouse embryonic stem (ES) cells to identify genes essential for self-renewal, and found 148 genes whose down-regulation caused differentiation, which supports the idea that regulatory networks controlling self-Renewal in stem cells may also be active in certain cancers. Expand