lacZ Reporter System as a Tool to Study Virulence Gene Regulation in Bacterial Pathogens.

@article{Aviv2018lacZRS,
  title={lacZ Reporter System as a Tool to Study Virulence Gene Regulation in Bacterial Pathogens.},
  author={Gili Aviv and Ohad Gal-Mor},
  journal={Methods in molecular biology},
  year={2018},
  volume={1734},
  pages={
          39-45
        }
}
β-galactosidase assay has been established as one of the most widely used reporters and can be effectually exploited to study promoter activity of Salmonella and other pathogens under various conditions. This method includes a preliminary stage of fusing the target promoter to a promoter-less lacZ gene encoding for the enzyme β-galactosidase. Supplementation of the synthetic ONPG substrate results in the accumulation of a chromogenic product proportionally to the activity of the fused promoter… CONTINUE READING

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