l-Ornithine:2-Oxoacid Aminotransferase from Squash (Cucurbita pepo, L.) Cotyledons: Purification and Properties.

@article{Lu1975lOrnithine2OxoacidAF,
  title={l-Ornithine:2-Oxoacid Aminotransferase from Squash (Cucurbita pepo, L.) Cotyledons: Purification and Properties.},
  author={T S Lu and M Mazelis},
  journal={Plant physiology},
  year={1975},
  volume={55 3},
  pages={502-6}
}
ORNITHINE: 2-oxoacid aminotransferase (EC 2.6.1.13) has been purified over 400-fold with a total recovery of 14% from acetone powders of cotyledons of germinating squash (Cucurbita pepo, L.) seedlings. The pH optimum of the transamination between l-ornithine and alpha-ketoglutarate is 8 and the Michaelis constants are 4.7 mm and 6.3 mm, respectively. The enzyme has a molecular weight of 48,000 as determined by gel filtration. The reaction is essentially specific for alpha-ketoglutarate as the… CONTINUE READING
4 Citations
8 References
Similar Papers

References

Publications referenced by this paper.
Showing 1-8 of 8 references

Ornithine transaminase from Curcurbita maxima cotyledons

  • H. J STRECKER
  • Phytochemistry
  • 1973

Biosynthesis of amino acids and related compounds

  • D. M. Greenberg
  • ' Metabolic Pathways
  • 1969

Conversion of ornithine into proline by enzymes from germinating peanut cotyledons

  • C. AND H. C. PITOT PERAINO
  • Phytochemistry
  • 1969

Mechanism of arginine biosynthesis in Chlamydomonas reinhardi . III . Purification and properties of acetylornithine - 6 - transaminase

  • J. AND G. DENES StiDI
  • Acta Biochim . Biophys . Acad . Sci . Hung .
  • 1967

Purification and properties of rat liver ornithine 5 - transaminase

  • J. AN'D G. DENES SiTDI
  • J . Biol . Chem .
  • 1965

Estimation of the molecular weights of proteins by Sephadex gel-filtration

  • P. ANDREWS
  • Biochem. J
  • 1964

Transamination of amino acids of the ornithine cycle in pea and wheat seedlings

  • A. L. FARR, AND R. J. RANDALL
  • Biochemistry
  • 1960

Similar Papers

Loading similar papers…