l‐Galactono‐γ‐lactone dehydrogenase from Arabidopsis thaliana, a flavoprotein involved in vitamin C biosynthesis

@article{Leferink2008lGalactonolactoneDF,
  title={l‐Galactono‐$\gamma$‐lactone dehydrogenase from Arabidopsis thaliana, a flavoprotein involved in vitamin C biosynthesis},
  author={Nicole G H Leferink and W. V. D. van den Berg and W. V. van Berkel},
  journal={The FEBS Journal},
  year={2008},
  volume={275}
}
l‐Galactono‐1,4‐lactone dehydrogenase (GALDH; ferricytochrome c oxidoreductase; EC 1.3.2.3) is a mitochondrial flavoenzyme that catalyzes the final step in the biosynthesis of vitamin C (l‐ascorbic acid) in plants. In the present study, we report on the biochemical properties of recombinant Arabidopsis thaliana GALDH (AtGALDH). AtGALDH oxidizes, in addition to l‐galactono‐1,4‐lactone (Km = 0.17 mm, kcat = 134 s−1), l‐gulono‐1,4‐lactone (Km = 13.1 mm, kcat = 4.0 s−1) using cytochrome c as an… Expand
l-Galactono-1,4-lactone dehydrogenase (GLDH) Forms Part of Three Subcomplexes of Mitochondrial Complex I in Arabidopsis thaliana*
TLDR
Evidence is increasing that GLDH represents a complex I assembly factor and two mitochondrial complexes of 470 and 420 kDa are identified, which further support a role of GLDH during complex I formation, which is based on its binding to specific assembly intermediates. Expand
Functional assignment of Glu386 and Arg388 in the active site of l‐galactono‐γ‐lactone dehydrogenase
TLDR
An essential Glu–Arg pair is identified in the active site of GALDH from Arabidopsis thaliana and shows high K m values for l‐galactono‐1,4‐lactone and low turnover rates. Expand
Communication between L–galactono–1,4–lactone dehydrogenase and cytochrome c
TLDR
The results point to a relatively low affinity GALDH–Cc interaction, similar for all partner redox states, involving protein–protein dynamic motions. Expand
Galactonolactone Dehydrogenase Requires a Redox-Sensitive Thiol for Optimal Production of Vitamin C1
TLDR
The indispensability of a redox-sensitive thiol provides a rationale why GALDH was designed as a dehydrogenase and not, like related aldonolactone oxidoreductases, as an oxidase. Expand
l-Galactono-1,4-lactone Dehydrogenase Is Required for the Accumulation of Plant Respiratory Complex I*
TLDR
The role of plant GLDH in both ascorbate biosynthesis and complex I accumulation is demonstrated, and l-galactono-1,4-lactone dehydrogenase (GLDH) is associated with the 800-kDa subcomplex but not with the holocomplex. Expand
Characterization of Two Arabidopsis L-Gulono-1,4-lactone Oxidases, AtGulLO3 and AtGulLO5, Involved in Ascorbate Biosynthesis.
TLDR
It is proposed that AsA synthesis through L-GulL in plants is regulated at the post-transcriptional level by limiting GulLO enzyme availability. Expand
Recent progress on the characterization of aldonolactone oxidoreductases.
TLDR
This review focuses on aldonolactone oxidoreductases, a subgroup of the vanillyl alcohol oxidase (VAO; EC 1.3.38) superfamily, enzymes that catalyze the terminal step in AsA biosynthesis in bacteria, protozoa, animals, and plants. Expand
Characterization of the galactono-1,4-lactone dehydrogenase from pepper fruits and its modulation in the ascorbate biosynthesis. Role of nitric oxide☆
TLDR
Results suggest that this modulation point of the ascorbate biosynthesis could be potentially used for biotechnological purposes to increase the vitamin C levels in pepper fruits. Expand
l-Galactono-1,4-lactone dehydrogenase is an assembly factor of the membrane arm of mitochondrial complex I in Arabidopsis
TLDR
The data suggest that GLDH is an assembly factor of the membrane arm of complex I, independent of the role of GLDH in ascorbate synthesis, as evidenced by the asCorbate-deficient mutant vtc2-1 accumulating wild-type levels ofcomplex I. Expand
Overexpression of L-galactono-1,4-lactone dehydrogenase (L-GalLDH) gene correlates with increased ascorbate concentration and reduced browning in leaves of Lactuca sativa L. after cutting
TLDR
Findings provide a first evidence of the role of endogenous AA as browning-preventing agent and the obtainment of T2 transgenic lettuce plants is a promising first step to further determine the specific mechanism by which AA acts as an anti-browning preservative. Expand
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l-Galactono-γ-lactone dehydrogenase (EC 1.3.2.3; GLDase), an enzyme that catalyzes the final step in the biosynthesis of l-ascorbic acid was purified 1693-fold from a mitochondrial extract ofExpand
L-Galactono-γ-lactone Dehydrogenase from Sweet Potato : Purification and cDNA Sequence Analysis
TLDR
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