in press-uncorrected proof Trafficking pathways of Cx 49-GFP in living mammalian cells

@inproceedings{Breidert2005inPP,
  title={in press-uncorrected proof Trafficking pathways of Cx 49-GFP in living mammalian cells},
  author={Stephanie Breidert and Ralf Jacob and Anaclet Ngezahayo and H. Kolb and Hassan Y Naim},
  year={2005}
}
In the present study we examined the trafficking pathways of connexin49 (Cx49) fused to green fluorescent protein (GFP) in polar and non-polar cell lines. The Cx49 gene was isolated from ovine lens by RT-PCR. Cx49 cDNA was fused to GFP and the hybrid cDNA was transfected into several cell lines. After transfection of Cx49GFP cDNA into HeLa cells, it was shown using the double whole-cell patch-clamp technique that the expressed fusion protein was still able to form conducting gap junction… CONTINUE READING

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