igital PCR provides absolute quantitation of viral load for an occult RNA virus ichard

Abstract

Using a multiplexed LNA-based Taqman assay, RT-digital PCR (RT-dPCR) was performed in a prefabricated microfluidic device that monitored absolute viral load in native and immortalized cell lines, overall precision of detection, and the absolute detection limit of an occult RNA virus GB Virus Type C (GBV-C). RT-dPCR had on average a 10% lower overall… (More)

3 Figures and Tables

Topics

  • Presentations referencing similar topics