iPS cells produce viable mice through tetraploid complementation

  title={iPS cells produce viable mice through tetraploid complementation},
  author={Xiao-Yang Zhao and Wei Li and Zhuo Lv and Lei Liu and Man Tong and Tang Hai and Jiejie Hao and Chang-long Guo and Qing-wen Ma and Liu Wang and Fanyi Zeng and Qi Zhou},
Since the initial description of induced pluripotent stem (iPS) cells created by forced expression of four transcription factors in mouse fibroblasts, the technique has been used to generate embryonic stem (ES)-cell-like pluripotent cells from a variety of cell types in other species, including primates and rat. It has become a popular means to reprogram somatic genomes into an embryonic-like pluripotent state, and a preferred alternative to somatic-cell nuclear transfer and somatic-cell fusion… 

Viable Fertile Mice Generated from Fully Pluripotent iPS Cells Derived from Adult Somatic Cells

Comparison of iPS cells from embryonic origin (MEF), progenitor cells (neural stem cells) or differentiated somatic cells (TTF) reveals that fully pluripotent developmental potential can be reached by each cell type, although with different induction efficiencies.

Generation of induced pluripotent stem cells from bovine embryonic fibroblast cells

The first establishment of bovine iPS cells using defined transcription factors and a modified culture medium is reported, which can be used to produce viable mice through tetraploid complementation and several mammalian species have been successfully generated.

Generation of Mice Derived from Induced Pluripotent Stem Cells

One method is described that produces fully pluripotent iPSCs that match ESCs in their developmental potential and efficiency of generating pups in TEC tests and is helpful for investigators wishing to compare the pluripotency of iPSC lines or establish the equivalence of different reprogramming methods.


iPS cell genesis being the result of a transient gene therapy mechanism, the transient epigenetic gene therapy arm of the proposed universal stem cell gene therepy platform is presented together with concurrent approaches mediated by protein transduction, mRNA transfection and small molecules.

Production of mice using iPS cells and tetraploid complementation

Details of the methods of generating iPS cell lines and subsequently producing full-term live animals through the tetraploid complementation assay are described; the procedure can be completed within 2 months.

Pluripotent Stem Cells and Reprogrammed Cells in Farm Animals

This review summarizes the present state of the art in the derivation and maintenance of pluripotent stem cells in domestic animals.

Pluripotency of induced pluripotent stem cells

It is proposed in this review that further comprehensive studies of both mouse and human iPS cells are required so that additional information will be available for evaluating the quality of human i PS cells.

Mice Cloned from Induced Pluripotent Stem Cells (iPSCs)1

Here, it is shown the successful production of viable cloned mice from inducible iPSCs through the nuclear transfer approach, and the efficiency is similar to that of using ESCs derived via normal fertilization.

Studies of Pluripotency in Embryonic Stem Cells and Induced Pluripotent Stem Cells

The first iPS mice in the world are reported, showing that fully reprogrammed iPSC using the four “Yamanaka factors” can be generated, and have similar developmental ability as ESCs, and will help improve the safety of pluripotent stem cell in clinical applications and increase the currently low efficiencies of their production.

Generation of germ cells in vitro in the era of induced pluripotent stem cells

These findings illustrate not only that iPSCs are developmentally similar to embryonic stem cells (ESCs), but also that somatic cells from adult tissues can produce gametes in vitro, that is, if they are reprogrammed into iPSC.



Induced Pluripotent Stem Cell Lines Derived from Human Somatic Cells

Somatic cell nuclear transfer allows trans-acting factors present in the mammalian oocyte to reprogram somatic cell nuclei to an undifferentiated state. We show that four factors (OCT4, SOX2, NANOG,

Induced Pluripotent Stem Cells Generated Without Viral Integration

This work generated mouse induced pluripotent stem cells from fibroblasts and liver cells by using nonintegrating adenoviruses transiently expressing Oct4, Sox2, Klf4, and c-Myc, providing strong evidence that insertional mutagenesis is not required for in vitro reprogramming.

Pluripotent stem cells induced from adult neural stem cells by reprogramming with two factors

It is shown that adult mouse neural stem cells express higher endogenous levels of Sox2 and c- myc than embryonic stem cells, and that exogenous Oct4 together with either Klf4 or c-Myc is sufficient to generate iPS cells from Neural stem cells.

Generation of Mouse Induced Pluripotent Stem Cells Without Viral Vectors

The production of virus-free iPS cells, albeit from embryonic fibroblasts, addresses a critical safety concern for potential use of i PS cells in regenerative medicine.

Induction of pluripotent stem cells from fibroblast cultures

Detailed methods and tips for the generation of induced pluripotent stem cells, named iPS cells, can be differentiated into three germ layers and committed to chimeric mice are described.

Generation of Pluripotent Stem Cells from Adult Mouse Liver and Stomach Cells

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piggyBac transposition reprograms fibroblasts to induced pluripotent stem cells

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Direct reprogramming of genetically unmodified fibroblasts into pluripotent stem cells

It is demonstrated that reprogrammed pluripotent cells can be isolated from genetically unmodified somatic donor cells solely based upon morphological criteria.