iPS cells produce viable mice through tetraploid complementation

@article{Zhao2009iPSCP,
  title={iPS cells produce viable mice through tetraploid complementation},
  author={Xiao-Yang Zhao and Wei Li and Zhuo Lv and Lei Liu and Man Tong and Tang Hai and Jiejie Hao and Chang-long Guo and Qing-wen Ma and Liu Wang and Fanyi Zeng and Qi Zhou},
  journal={Nature},
  year={2009},
  volume={461},
  pages={86-90}
}
Since the initial description of induced pluripotent stem (iPS) cells created by forced expression of four transcription factors in mouse fibroblasts, the technique has been used to generate embryonic stem (ES)-cell-like pluripotent cells from a variety of cell types in other species, including primates and rat. It has become a popular means to reprogram somatic genomes into an embryonic-like pluripotent state, and a preferred alternative to somatic-cell nuclear transfer and somatic-cell fusion… 

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Somatic cell nuclear transfer allows trans-acting factors present in the mammalian oocyte to reprogram somatic cell nuclei to an undifferentiated state. We show that four factors (OCT4, SOX2, NANOG,

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TLDR
This work generated mouse induced pluripotent stem cells from fibroblasts and liver cells by using nonintegrating adenoviruses transiently expressing Oct4, Sox2, Klf4, and c-Myc, providing strong evidence that insertional mutagenesis is not required for in vitro reprogramming.

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TLDR
It is shown that adult mouse neural stem cells express higher endogenous levels of Sox2 and c- myc than embryonic stem cells, and that exogenous Oct4 together with either Klf4 or c-Myc is sufficient to generate iPS cells from Neural stem cells.

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TLDR
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TLDR
Detailed methods and tips for the generation of induced pluripotent stem cells, named iPS cells, can be differentiated into three germ layers and committed to chimeric mice are described.

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TLDR
Genetic lineage tracings suggest that iPS cells are generated by direct reprogramming of lineage-committed somatic cells and that retroviral integration into specific sites is not required.

piggyBac transposition reprograms fibroblasts to induced pluripotent stem cells

TLDR
It is shown that the individual PB insertions can be removed from established iPS cell lines, providing an invaluable tool for discovery, and the traceless removal of reprogramming factors joined with viral 2A sequences delivered by a single transposon from murine iPS lines is demonstrated.

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TLDR
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