hnRNP A1 binds promiscuously to oligoribonucleotides: utilization of random and homo-oligonucleotides to discriminate sequence from base-specific binding.

@article{AbdulManan1996hnRNPAB,
  title={hnRNP A1 binds promiscuously to oligoribonucleotides: utilization of random and homo-oligonucleotides to discriminate sequence from base-specific binding.},
  author={Norzehan Abdul-Manan and Kenneth R. Williams},
  journal={Nucleic acids research},
  year={1996},
  volume={24 20},
  pages={4063-70}
}
To understand the range of possible and probable A1 functions in pre-mRNA biogenesis, it is important that we quantify the relative ability (or inability) of A1 to bind high affinity RNA target sequences and/or structures. Using a fluorescence competition assay we have determined apparent binding affinities for a wide range of 20mer oligos containing putative and possible A1 targets including the high affinity 'winner' sequence identified by selection/amplification [Burd,C.G and Dreyfuss,G… CONTINUE READING