hUBC9 associates with MEKK1 and type I TNF-alpha receptor and stimulates NFkappaB activity.

Abstract

hUBC9, an E2 ubiquitin conjugating enzyme, was identified by yeast two-hybrid screening and coprecipitation studies to interact with MEKK1 and the type I TNF-alpha receptor, respectively. Because both of these proteins regulate NFkappaB activity, the role of hUBC9 in modulating NFkappaB activity was investigated. Overexpression of hUBC9 in HeLa cells stimulated the activity of NFkappaB as determined by NFkappaB reporter and IL-6 secretion assays. hUBC9 also synergized with MEKK1 to activate NFkappaB reporter activity. Thus, hUBC9 modulates NFkappaB activity which, at least in part, can be attributed to its interaction with MEKK1 and the type I TNF-alpha receptor.

Statistics

050'04'06'08'10'12'14'16
Citations per Year

216 Citations

Semantic Scholar estimates that this publication has 216 citations based on the available data.

See our FAQ for additional information.

Cite this paper

@article{Saltzman1998hUBC9AW, title={hUBC9 associates with MEKK1 and type I TNF-alpha receptor and stimulates NFkappaB activity.}, author={Artur Saltzman and George H. Searfoss and Christophe Marcireau and Mariam Stone and Roseanne A Ressner and Ronald G. Munro and Chris R. Franks and J S D'Alonzo and Bruno Tocqu{\'e} and Momodou Cherno Jaye and Yuri Ivashchenko}, journal={FEBS letters}, year={1998}, volume={425 3}, pages={431-5} }