gltBDF operon of Escherichia coli.

  title={gltBDF operon of Escherichia coli.},
  author={Irene Casta{\~n}o and Fernando Bastarrachea and Alejandra A Covarrubias},
  journal={Journal of bacteriology},
  volume={170 2},
A 2.0-kilobase DNA fragment carrying antibiotic resistance markers was inserted into the gltB gene of Escherichia coli previously cloned in a multicopy plasmid. Replacement of the chromosomal gltB+ gene by the gltB225::omega mutation led to cells unable to synthesize glutamate synthase, utilize growth rate-limiting nitrogen sources, or derepress their glutamine synthetase. The existence of a gltBDF operon encoding the large (gltB) and small (gltD) subunits of glutamate synthase and a regulatory… CONTINUE READING
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