gamma Ray induced deoxyribonucleic acid strand breaks. 3' Glycolate termini.

Abstract

Gamma irradiation of DNA in aqueous solution produces single strand breaks with two types of 3'-termini. These termini have been identified as a simple phosphoryl group and a glycolate moiety attached to the 3'-phosphoryl group via the 2"-OH of glycolic acid. Irradiation of 5'-end labeled poly(dG) produces compounds that comigrate with authentic 3',5'-dGDP and 2'-deoxyguanosine-3'-(phospho-2"-O-glycolic acid-5'-monophosphate by thin layer chromatography and polyacrylamide gel electrophoresis. Irradiation of poly(dGdC) followed by digestion to 3'-nucleotides produces 2'-deoxyguanosine-3'-(phospho-2"-O-glycolic acid). The presence of glycolate termini at some strand breaks may affect intracellular repair of these lesions.

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@article{Henner1983gammaRI, title={gamma Ray induced deoxyribonucleic acid strand breaks. 3' Glycolate termini.}, author={William David Henner and Lily O Rodr{\'i}guez and Sidney M Hecht and William A. Haseltine}, journal={The Journal of biological chemistry}, year={1983}, volume={258 2}, pages={711-3} }