gTME for improved adaptation of Saccharomyces cerevisiae to corn cob acid hydrolysate.

  title={gTME for improved adaptation of Saccharomyces cerevisiae to corn cob acid hydrolysate.},
  author={Hongmei Liu and Kai Sui Liu and Ming Yan and Lin Xu and Pingkai Ouyang},
  journal={Applied biochemistry and biotechnology},
  volume={164 7},
Global transcription machinery engineering was employed to engineer xylose metabolism, tolerance and adaptation to lignocellulosic hydrolysates. Mutation of the transcription factor spt15 was introduced by error-prone PCR, then transformed the recombinant plasmid pYX212-spt15 into Saccharomyces cerevisiae YPH499 which was not able to grow on xylose, and screened on media using lignocellulosic hydrolysates as the sole carbon source. The maximum sugar yield was obtained by the hydrolysis form… CONTINUE READING
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