epiGBS: reference-free reduced representation bisulfite sequencing

  title={epiGBS: reference-free reduced representation bisulfite sequencing},
  author={Thomas P. van Gurp and Niels C. A. M. Wagemaker and Bj{\"o}rn Wouters and Philippine Vergeer and Joop N J Ouborg and Koen J F Verhoeven},
  journal={Nature Methods},
We describe epiGBS, a reduced representation bisulfite sequencing method for cost-effective exploration and comparative analysis of DNA methylation and genetic variation in hundreds of samples de novo. This method uses genotyping by sequencing of bisulfite-converted DNA followed by reliable de novo reference construction, mapping, variant calling, and distinction of single-nucleotide polymorphisms (SNPs) versus methylation variation (software is available at https://github.com/thomasvangurp… CONTINUE READING
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