eIF4G dramatically enhances the binding of eIF4E to the mRNA 5'-cap structure.

  title={eIF4G dramatically enhances the binding of eIF4E to the mRNA 5'-cap structure.},
  author={Ashkan Haghighat and Nahum Sonenberg},
  journal={The Journal of biological chemistry},
  volume={272 35},
The cap structure, m7GpppN, is present at the 5'-end of all eukaryotic cellular (except organellar) mRNAs. Initiation of translation is mediated by the multisubunit initiation factor eIF4F, which binds the cap structure via its eIF4E subunit and facilitates the binding of mRNA to ribosomes. Here, we used recombinant proteins to reconstitute the cap recognition activity of eIF4F in vitro. We demonstrate that the interaction of eIF4E with the mRNA 5'-cap structure is dramatically enhanced by… CONTINUE READING
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