delta(6)-desaturase of Mucor rouxii with high similarity to plant delta(6)-desaturase and its heterologous expression in Saccharomyces cerevisiae.

@article{Laoteng2000delta6desaturaseOM,
  title={delta(6)-desaturase of Mucor rouxii with high similarity to plant delta(6)-desaturase and its heterologous expression in Saccharomyces cerevisiae.},
  author={Kobkul Laoteng and R Mannontarat and Morakot Tanticharoen and Supapon Cheevadhanarak},
  journal={Biochemical and biophysical research communications},
  year={2000},
  volume={279 1},
  pages={17-22}
}
Gamma-linolenic acid (GLA, gamma-C18:3) is an essential fatty acid that plays a vital role in biological structures and cellular functions. Based on available sequence information and using polymerase chain reaction (PCR) technique, we cloned from the fungus Mucor rouxii the entire coding sequence of a delta(6)-desaturase enzyme, which is responsible for the transformation of linoleic acid into GLA. The deduced amino acid sequence of M. rouxii gene showed the highest homology with the plant… CONTINUE READING
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