d.

@article{Zhou2021d,
  title={d.},
  author={Danna Zhou and Shuangshuang Wang and Keli Yang and X. Liu and W. Liu and R. Guo and W. Liang and F. Yuan and Zewen Liu and Ting Gao and Yongxiang Tian},
  journal={Microbial pathogenesis},
  year={2021},
  pages={
          104724
        }
}
Japaneses encephalitis (JE) is most common zoonoses caused by Japanese encephalitis virus (JEV) with a high mortality and disability rate. To take timely preventive and control measures, early and rapid detection of JE RNA is necessary. But due to characteristic brief and low viraemia, JE RNA detection remains challenging. In this study, a real-time nucleic acid sequence-based amplification (RT-NASBA) was developed for rapid and simultaneous detection of JEV. Four pairs of primer were designed… Expand
Diagnostic techniques for COVID-19 and new developments
TLDR
The reverse transcription-polymerase chain reaction (RT-PCR) is the most routinely used method until now to detect SARS-CoV-2 infections, but several other faster and accurate assays are being developed for the diagnosis of COVID-19 aiming to control the spread of infection through the identification of patients and immediate isolation. Expand
Simultaneous detection of duck circovirus and novel goose parvovirus via SYBR green I-based duplex real-time polymerase chain reaction analysis
  • Yong Wang, Yongqiu Cui, +4 authors Yong-dong Li
  • Medicine, Biology
  • Molecular and Cellular Probes
  • 2020
TLDR
This duplex real-time PCR assay offers advantages over other tests, such as rapid, sensitive, specific, and reliable detection of both viruses in a single sample, which enables the quantitative detection of DuCV and NGPV in clinical samples. Expand
One-step multiplex real-time RT-PCR for detection and typing of dengue virus.
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This study developed a TaqMan probe-based, one-step real-time reverse transcriptase-polymerase chain reaction (RT-PCR) system for detection and serotyping of dengue virus (DENV) and indicated that this system is specific for DENV-1, 2, 3, and 4 and does not react with other viruses. Expand
A Streamlined Approach to Rapidly Detect SARS-CoV-2 Infection Avoiding RNA Extraction: Workflow Validation
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This work developed a fast and cost-effective viral genome isolation method that, combined with quantitative RT-PCR assay, detects SARS-CoV-2 RNA in patient samples and has been characterized in 60 COVID-19-infected patients. Expand
Immunopathology, host-virus genome interactions, and effective vaccine development in SARS-CoV-2
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The aim of this review article is to explore recent updates on SARS-COV-2 in the context of pathogenesis during disease progression, and innate acquired mechanisms of defense, including advances in diagnostics, susceptibility, and severity of host-virus genome interactions. Expand
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The data support the consideration that detection of the N2 gene at high Ct needs to be interpreted with caution, suggesting that collaboration between virologists and clinicians is important for better understanding of results. Expand
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The COVID-19 pandemic remains a significant problem involving health systems worldwide and real-time reverse-transcriptase polymerase-chain-reaction (rRT-PCR) is considered the gold standard, although the occurrence of falsely negative results in symptomatic patients and/or having a positive CT scan remains a challenge. Expand
Diagnosis and treatment of coronavirus disease 2019 (COVID-19): Laboratory, PCR, and chest CT imaging findings
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This review focuses on currently available information on the etiology, clinical symptoms, diagnosis, and mechanism of action of COVID-19 and presents an overview of diagnostic approaches and treatment of this disease according to available findings. Expand
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The results show that the surrogate virus-neutralization test (sVNT) holds promise in detecting RBD-binding antibodies in multiple species, however it lacked sensitivity to detect low titers. Expand
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