cDNA cloning of thylakoid-bound ascorbate peroxidase in pumpkin and its characterization.


A cDNA for thylakoid-bound ascorbate peroxidase of pumpkin was cloned and characterized. Thylakoid-bound ascorbate peroxidase had a high similarity to cytosolic ascorbate peroxidases, and the precursor contained a transit peptide to chloroplasts at its amino-terminus and a putative membrane-spanning region at its carboxy-terminus. 


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