cDNA cloning of growth hormone from giant panda (Ailuropoda melanoleuca) and its expression in Escherichia coli.

@article{Liao2003cDNACO,
  title={cDNA cloning of growth hormone from giant panda (Ailuropoda melanoleuca) and its expression in Escherichia coli.},
  author={Ming Juan Liao and Mu Yuan Zhu and Xu Song Zheng and Zhi He Zhang and An Ju Zhang},
  journal={Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology},
  year={2003},
  volume={135 1},
  pages={109-16}
}
A cDNA encoding Ailuropoda melanoleuca growth hormone (AmGH) was isolated from pituitary total RNA using RT-PCR and expressed in Escherichia coli. This is the first report of a GH nucleotide and amino acid (aa) sequence from giant panda. The open reading frame of AmGH (651 bp) encodes a precursor of 216 aa comprising a 26 aa signal peptide and a 190 aa mature protein with four cysteine residues similar to the typical primary structure of mammalian GH precursor. AmGH shares a high degree of… CONTINUE READING