cDNA cloning of bovine liver dihydropyrimidine dehydrogenase.

  title={cDNA cloning of bovine liver dihydropyrimidine dehydrogenase.},
  author={N. Albin and Martin R. Johnson and Robert B. Diasio},
  journal={DNA sequence : the journal of DNA sequencing and mapping},
  volume={6 4},
Dihydropyrimidine dehydrogenase (DPD), the initial and rate-limiting enzyme in pyrimidine catabolism, has recently been purified to homogeneity from several species. In the present study the molecular cloning of DPD with isolation of a cDNA coding for bovine liver DPD is reported using polymerase chain reaction (PCR) methodology. Known amino acid sequence from purified bovine DPD was used to initially design mixed oligonucleotide primers for amplification of a cDNA fragment (65 base pairs… CONTINUE READING