cDNA cloning and induction of tyrosine hydroxylase gene from the diamondback moth, Plutella xylostella.

@article{Hwang2010cDNACA,
  title={cDNA cloning and induction of tyrosine hydroxylase gene from the diamondback moth, Plutella xylostella.},
  author={Sejung Hwang and Saeyoull Cho and Yong Chul Park},
  journal={Archives of insect biochemistry and physiology},
  year={2010},
  volume={75 2},
  pages={
          107-20
        }
}
We cloned a full-length tyrosine hydroxylase cDNA from the integument of the diamondback moth, Plutella xylostella. In the phylogenetic tree, tyrosine hydroxylase (PxTH) clustered with the other lepidopteran THs. Serine residues in the PxTH sequence, namely Ser(24), Ser(31), Ser(35), Ser(53), and Ser(65), were predicted to be the target sites for phosphorylation based on PROSITE analysis. In particular, Ser(35) of PxTH is highly conserved across a broad phylogenetic range of animal taxa… 
View on PubMed
doi.org
2 Citations
Background Citations
1

Figures and Tables from this paper

2 Citations

Parasitization by Cotesia chilonis Influences Gene Expression in Fatbody and Hemocytes of Chilo suppressalis

The hypothesis that parasitization by a braconid wasp, Cotesia chilonis, influences the expression of genes responsible for development, metabolism and immune functions in the fatbody and hemocytes of its host, Chilo suppressalis, is supported.

Origin and Evolution of the Neuroendocrine Control of Reproduction in Vertebrates, with Special Focus on Genome and Gene Duplications.

The paper reviews, from molecular and functional perspectives, the evolution across vertebrate radiation of some key-actors of the vertebrates neuroendocrine control of reproduction, and traces back their origin along the vertebrate lineage and in other metazoa before the emergence of vertebrates.

References

SHOWING 1-10 OF 39 REFERENCES

Characterization of tyrosine hydroxylase from Manduca sexta.

Induction of tyrosine hydroxylase gene expression by bacteria in the fat body of eri-silkworm, Samia cynthia ricini.

Laccase 2 is the phenoloxidase gene required for beetle cuticle tanning.

The hypothesis that two isoforms of laccase 2 generated by alternative splicing catalyze larval, pupal, and adult cuticle tanning in Tribolium is supported.

Isolation and characterization of the gene for drosophila tyrosine hydroxylase

Identification of immunorelevant genes from greater wax moth (Galleria mellonella) by a subtractive hybridization approach.

Insect cytokine, growth‐blocking peptide, is a primary regulator of melanin‐synthesis enzymes in armyworm larval cuticle

This study demonstrated that tyrosine hydroxylase (TH) expression is activated synchronously with DDC, and enhancement of DDC and TH transcription is due to an increase in cyotoplasmic Ca2+, which is induced by the insect cytokine, growth‐blocking peptide (GBP).

Structure of the human tyrosine hydroxylase gene: alternative splicing from a single gene accounts for generation of four mRNA types.

Four types of human TH mRNA are produced through alternative splicing from a single gene: the alternative use of two donor sites in the first exon, and the inclusion/exclusion of the second exon.

Differential Regulation of Drosophila Tyrosine Hydroxylase Isoforms by Dopamine Binding and cAMP-dependent Phosphorylation*

It is proposed that tissue-specific alternative splicing of TH has a functional role for differential regulation of dopamine biosynthesis in the nervous and non-nervous tissues of insects.

A novel and major isoform of tyrosine hydroxylase in Drosophila is generated by alternative RNA processing.

Bacterial-injection-induced syntheses of N-beta-alanyldopamine and Dopa decarboxylase in the hemolymph of coleopteran insect, Tenebrio molitor larvae.

Injection of Escherichia coli into larvae of the coleopteran Tenebrio molitor resulted in the appearance of a dopamine-like substance on the electrochemical detector, which was determined to be N-beta-alanyldopamine, which might be used as a substrate by phenoloxidase during melanin synthesis in the humoral defense response or the melanotic encapsulation reaction of the cellular defense response.