cDNA cloning and gene expression of ascorbate oxidase in tobacco

  title={cDNA cloning and gene expression of ascorbate oxidase in tobacco},
  author={Naohiro Kato and Muneharu Esaka},
  journal={Plant Molecular Biology},
A cDNA clone for ascorbate oxidase (AAO) has been isolated from a cDNA library of tobacco (Nicotiana tabacum) cells. The identity of the amino acid sequence deduced from tobacco AAO cDNA to that from pumpkin AAO cDNA was 68%, which was much lower than the identity (80%) between pumpkin and cucumber AAO. AAO activity in tobacco cells was much lower than that in pumpkin cells, whereas the immunoreactive protein in tobacco cells was more abundant than that in pumpkin cells. We suppose that AAO… CONTINUE READING
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