cDNA cloning and functional expression of α-glucosidase from Mortierella alliacea

@article{Tanaka2003cDNACA,
  title={cDNA cloning and functional expression of α-glucosidase from Mortierella alliacea},
  author={Yoshiyuki Tanaka and Tsunehiro Aki and Koji Ishihara and Seiji Kawamoto and Seiko Shigeta and Ken-ichiro Ono},
  journal={Applied Microbiology and Biotechnology},
  year={2003},
  volume={62},
  pages={202-209}
}
We recently purified an α-glucosidase comprising 61-kDa and 31-kDa subunits from the fungus Mortierella alliacea and characterized its soluble starch-hydrolyzing activity. Here, the cDNA coding for this enzyme was cloned, revealing that it encodes a single polypeptide of 1,053 amino acids, with a calculated molecular mass of 117 kDa. Comparison between the deduced amino acid sequence and the partial sequences of the purified enzyme suggested that an immature protein can be converted into the… CONTINUE READING
4 Citations
28 References
Similar Papers

References

Publications referenced by this paper.
Showing 1-10 of 28 references

Production of arachidonic acid by filamentous fungus, Mortierella alliacea strain YN-15

  • T Aki, Y Nagahata, +9 authors O Suzuki
  • J Am Oil Chem Soc 78:599–604
  • 2001
Highly Influential
7 Excerpts

Novel a - glucosidase from Aspergillus nidulans with strong transglycosylation activity

  • A Kimura, M Takata, +6 authors S Chiba
  • Appl Environ Microbiol
  • 2002

Purification and characterization of a novel fungal a - glucosidase from Mortierella alliacea with high starch - hydrolytic activity

  • Y Yamasaki, T Miyake, Y Suzuki
  • Biosci Biotechnol Biochem
  • 2002

Purification and characterization of a novel fungal a-glucosidase from Mortierella alliacea with high

  • K Ono, O Suzuki
  • 2002

Similar Papers

Loading similar papers…