cDNA cloning and expression of Brassica napus enoyl-acyl carrier protein reductase in Escherichia coli

@article{Kater1991cDNACA,
  title={cDNA cloning and expression of Brassica napus enoyl-acyl carrier protein reductase in Escherichia coli},
  author={Martin M. Kater and Gregory M. Koningstein and H. J. Nijkamp and A. R. Stuitje},
  journal={Plant Molecular Biology},
  year={1991},
  volume={17},
  pages={895-909}
}
The onset of storage lipid biosynthesis during seed development in the oilseed crop Brassica napus (rape seed) coincides with a drastic qualitative and quantitative change in fatty acid composition. During this phase of storage lipid biosynthesis, the enzyme activities of the individual components of the fatty acid synthase system increase rapidly. We describe a rapid and simple purification procedure for the plastidlocalized NADH-dependent enoyl-acyl carrier protein reductase from developing B… CONTINUE READING
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